EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.1.145 | gene dnk, isolated from a phage library, DNA and amino acid sequence determination and analysis, recombinant expression of randomly mutated genes for deoxyribonucleoside kinase in Escherichia coli strain BL21, recombinant expression of catalytically active His10-tagged mutant 4 enzyme in Spodoptera frugiperda SF9 cells | Drosophila melanogaster |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.145 | EDTA | - |
Drosophila melanogaster |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.145 | Mg2+ | required | Drosophila melanogaster |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.145 | ATP + 2'-deoxyribonucleoside | Drosophila melanogaster | - |
ADP + 2'-deoxyribonucleoside 5'-phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.1.145 | Drosophila melanogaster | Q9XZT6 | gene dnk | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.1.145 | recombinant His10-tagged enzyme from Spodoptera frugiperda SF9 cells by nickel affinity chromatography and gel filtration | Drosophila melanogaster |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.145 | ATP + 2'-deoxycytidine | - |
Drosophila melanogaster | ADP + 2'-deoxycytidine 5'-phosphate | - |
? | |
2.7.1.145 | ATP + 2'-deoxyribonucleoside | - |
Drosophila melanogaster | ADP + 2'-deoxyribonucleoside 5'-phosphate | - |
? | |
2.7.1.145 | ATP + 2-hydroxy-2'-deoxyadenosine | preferred substrate for enzyme mutant 4 | Drosophila melanogaster | ADP + 2-hydroxy-2'-deoxyadenosine 5'-monophosphate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.1.145 | Dm-dNK | - |
Drosophila melanogaster |
2.7.1.145 | multisubstrate deoxyribonucleoside kinase | - |
Drosophila melanogaster |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.1.145 | 37 | - |
assay at | Drosophila melanogaster |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.1.145 | 8 | - |
assay at | Drosophila melanogaster |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.145 | ATP | - |
Drosophila melanogaster |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.7.1.145 | additional information | enzyme identification using a mutagenic nucleoside analogue 2-hydroxy-2'-deoxyadenosine 5'-monophosphate, overview. Method development and evaluation for in vivo evolution selection method for active deoxyribonucleoside kinase proteins, including selection of antibiotic-resistant colonies resulting frommutations by phosphorylated 2-hydroxy-deoxyadenosine and the subsequent isolation of the plasmid DNAs. Fifteen Dm-dNK mutants selected after the seventh and eighth evolutioncycles are actually active in vivo, one shows activity similar to the wild-typ enzyme | Drosophila melanogaster |